ABSTRACT

Diffquick dying is one the methods of cytological dying. The diffquick method’s components
is methylene blue. Methylene blue is toxic and non-biogradable so it requires natural dyes
that do not have harmful properties for the body. Clitoria ternatea is a natural colorant
that has an anthocyanin content and produces blue pigment and has been tested to color
the nucleus of epithelial cells. The purpose of research for the influence of temperature
factors and long storage of the solution of clit extract as a nutarl colour subtitutes for
methylene blue in the coloring of nucleus cells diffquick method. Research design is
experimental with purposive sampling. The research was conducted in January-March
2024 at the sitohistotechnology laboratory. The sample is a buccal smear and coloring
using diffquick method. The test was conducted for five days with a sample of 15
preparations. The Mann whitney (sig.) test result 1.0>0.05 or H0 was received so there was
no difference in the core color power of the cell with a 16% concentration of oak flower
extract stored at 7-110C dan 250C as a substitute for the methylene blue dye in diffquick
dyeing. This shows that there is no difference in the color power of the cell from the solution
of the butterfly pea extract that are stored at showcase and room temperature.

Key word : Butterflye pea flower, extract, diffquick, methylene blue, buccal smear.